| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Davis, Alexander J (17)
|
OHDCST |
4:49.49 |
4:48.82 |
-0.67 |
| 2 |
Stump, Zachary M (17)
|
OHWAC |
|
4:50.13 |
- |
| 3 |
Brown, Emily M (14)
|
OHWAC |
4:57.98 |
4:57.17 |
-0.81 |
| 4 |
Rowe, Blake M (15)
|
MIGTBY |
5:25.16 |
5:03.43 |
-21.73 |
| 5 |
Nadur, Benjamin P (14)
|
OHWAC |
5:19.61 |
5:05.70 |
-13.91 |
| 6 |
Donovan, Liam M (14)
|
OHDCST |
5:23.89 |
5:07.86 |
-16.03 |
| 7 |
Randolph, Graham J (17)
|
OHUDCS |
5:25.00 |
5:09.03 |
-15.97 |
| 8 |
Burnard, John E (18)
|
OHBGSC |
4:54.02 |
5:09.28 |
15.26 |
| 9 |
Andrews, Elizabeth G (18)
|
OHWAC |
4:58.01 |
5:13.06 |
15.05 |
| 10 |
Klein, Tyler A (13)
|
OHBKYS |
5:45.06 |
5:18.58 |
-26.48 |
| 11 |
Wu, Alex X (13)
|
OHDCST |
5:53.56 |
5:24.25 |
-29.31 |
| 12 |
Van Frayen, Brody M (14)
|
OHBKYS |
5:34.76 |
5:28.02 |
-6.74 |
| 13 |
Ellsworth, Emily T (17)
|
OHWAC |
5:23.14 |
5:29.05 |
5.91 |
| 14 |
Hensley, Max R (15)
|
OHLYST |
5:25.99 |
5:30.85 |
4.86 |
| 15 |
Hensley, Mia S (17)
|
OHLYST |
5:06.99 |
5:32.32 |
25.33 |
| 16 |
Randolph, Helen M (13)
|
OHDCST |
5:26.77 |
5:36.36 |
9.59 |
| 17 |
Ruff, Elizabeth J (17)
|
OHWAC |
5:47.98 |
5:37.58 |
-10.40 |
| 18 |
Robie, Jessica L (13)
|
OHFMST |
5:33.00 |
5:40.04 |
7.04 |
| 19 |
Brayton, Elyse F (15)
|
MIGTBY |
6:11.41 |
5:44.27 |
-27.14 |
| 20 |
Crumbley, Kiara L (17)
|
OHEP |
5:56.54 |
5:45.72 |
-10.82 |
| 21 |
Stearns, Ashby C (13)
|
OHDCST |
6:02.21 |
5:45.92 |
-16.29 |
| 22 |
Grubbs, Caleb T (15)
|
OHBGSC |
6:30.42 |
5:47.70 |
-42.72 |
| 23 |
Van Buskirk, Emmett J (13)
|
OHDCST |
5:29.38 |
5:48.20 |
18.82 |
| 24 |
Knodel, Alexandria J (17)
|
OHWAC |
5:39.35 |
5:50.10 |
10.75 |
| 25 |
Smith, Kailyn (17)
|
OHUBKY |
5:55.83 |
5:50.56 |
-5.27 |
| 26 |
Bills, Avery D (15)
|
MIGTBY |
5:58.70 |
5:54.07 |
-4.63 |
| 27 |
Sanders, Natalie A (14)
|
OHFMST |
6:35.64 |
5:56.10 |
-39.54 |
| 28 |
Syrek, Mallory E (13)
|
OHFMST |
5:55.00 |
5:57.05 |
2.05 |
| 29 |
Blakely, Madeline A (18)
|
OHBGSC |
5:49.15 |
5:57.53 |
8.38 |
| 30 |
Randall, Melaina B (14)
|
OHBKYS |
6:01.36 |
5:59.44 |
-1.92 |
| 31 |
Smith, Jillian A (13)
|
OHDCST |
5:57.44 |
6:06.37 |
8.93 |
| 32 |
Garrett, Haylee A (13)
|
OHLYST |
6:09.99 |
6:16.17 |
6.18 |
| 33 |
Hildebrand, Kate E (14)
|
OHFMST |
5:59.00 |
6:18.44 |
19.44 |
| 34 |
Swartz, Kristin M (17)
|
OHKCST |
5:50.00 |
6:19.22 |
29.22 |
| 35 |
Adams, Wilson P (13)
|
OHDCST |
5:56.26 |
6:21.07 |
24.81 |
| 36 |
Layne, Margaret S (15)
|
OHBGSC |
6:30.20 |
6:25.78 |
-4.42 |